Determination of anabolic-androgenic steroid adulterants in counterfeit drugs by UHPLC-MS/MS


Anabolic–androgenic steroids (AASs) have been illegally used in counterfeit drugs to improve the performance of athletes. In addition, AASs have been used for cosmetic purpose by non-athletes. To determine the presence of 26 AASs, an analysis method using ultra-liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) was developed and validated. The validated method was applied to 19 counterfeit drugs collected from the Internet and off-line markets during 2014. Nearly 50% (9/19) of the samples contained one of these 26 AASs. In addition, the concentration ranges of the AASs ranged from 0.09 to 119,228.57 mg/kg in the suspected samples. The determined AASs primarily consisted of testosterone and testosterone 17-propionate (26%) followed by boldenone (21%). These results indicate the adulteration of over-the-counter counterfeit drugs, and the continuous monitoring of counterfeit drugs or dubious dietary supplements containing anabolic steroids is warranted.


Autor / Fonte:So-Hyun Cho, Hyoung Joon Park, Ji Hyun Lee, Jung-Ah Do, Seok Heo, Jeong Hwa Jo, Sooyeul Cho Journal of Pharmaceutical and Biomedical Analysis 2015 March 28, 111: 138-146