Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal

Abstract

Objective

We tested the hypothesis that skeletal muscle of endurance-trained male runners would exhibit elevated autophagy and mitophagy markers, which would be associated with greater metabolic flexibility following a high-fat meal (HFM).

Methods

Muscle biopsies were collected to determine differences in autophagy and mitophagy protein markers and metabolic flexibility under fasting conditions and 4 h following a HFM between endurance-trained male runners (n = 10) and sedentary, non-obese controls (n = 9).

Results

Maximal oxygen consumption (ml·kg·min −1 ) was approximately 50% higher (p < 0.05) in endurance-trained runners compared with sedentary controls (65.8 ± 2.3 and 43.1 ± 3.4, respectively). Autophagy markers were similar between groups. Mitophagy and mitochondrial dynamics protein markers were significantly higher in skeletal muscle of endurance-trained runners compared with sedentary controls in the fasted state, although unaffected by the HFM. Skeletal muscle metabolic flexibility was similar between groups when fasted (p > 0.05), but increased in response to the HFM in endurance-trained athletes only (p < 0.005). Key mitophagy markers, phospho-Pink1 Thr257 and phospho-Parkin S65 (r = 0.64, p < 0.005), and phospo-Parkin Ser65 and phospho-Drp1Ser616 (r = 0.70, p < 0.05) were correlated only within the endurance-trained group. Autophagy and mitophagy markers were not correlated with metabolic flexibility.

Conclusion

In summary, mitophagy may be enhanced in endurance-trained runners based on elevated markers of mitophagy and mitochondrial dynamics. The HFM did not alter autophagy or mitophagy in either group. The absence of a relationship between mitophagy markers and metabolic flexibility suggests that mitophagy is not a key determinant of metabolic flexibility in a healthy population, but further investigation is warranted.

Highlights

 

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    Basal autophagy is similar in skeletal muscle of endurance-trained and sedentary males.

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    Trained and sedentary skeletal muscle autophagy is unaltered following high-fat meal.

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    Mitophagy activity is greater in endurance-trained than sedentary skeletal muscle.

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    Greater mitophagy is related to increased metabolic flexibility after high-fat meal.

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    Trained and sedentary skeletal muscle mitophagy is unaltered following high-fat meal.


Autor / Fonte:Michael D Tarpey, Kevin P Davy, Ryan P McMillan, Suzanne M Bowser, Tanya M Halliday, Nabil E Boutagy, Brenda M Davy, Madlyn I Frisard, Matthew W Hulver Molecular Metabolism 2017 October 21
Link: https://www.clinicalkey.com/service/content/pdf/watermarked/1-s2.0-S2212877817306646.pdf?locale=pt_BR